Концентрации BDNF и нейропептидов в крови как потенциальные биомаркеры поведенческих нарушений у крыс с опытом ультразвукового пренатального стресса

В экспериментальном исследовании с целью оценки концентрации BDNF, α-МСГ, β-эндорфина, нейротензина, окситоцина и субстанции Р в плазме крови крыс с опытом ультразвукового пренатального стресса и выявления взаимосвязи концентраций с поведением крыс на беременных самок непрерывно воздействовали ультразвуком переменной частоты в течение всего гестационного периода. Оценивали поведение полученного потомства в тестах «Открытое поле», «Приподнятый крестообразный лабиринт», «Социальное взаимодействие», тесте на ангедонию и тесте «Принудительное плавание». Оценка концентраций проводилась методами иммуноферментного и мультиплексного анализов. Ультразвуковой пренатальный стресс снизил двигательную и исследовательскую, а также нормальную социальную активность, усилил тревожность и агрессивное социальное поведение у крыс (изменения были более выражены у самцов), увеличил концентрацию окситоцина в плазме самок. Выявлены различия между полами: у контрольных самцов была отмечена более низкая концентрация α-МСГ, чем у контрольных самок, что не наблюдалось у потомства с пренатальным стрессом; концентрация субстанции Р была снижена у самцов с пренатальным стрессом по сравнению с самками с пренатальным стрессом, что не наблюдалось в контрольной группе. Полученные данные можно использовать при изучении предикторов поведенческих нарушений у человека.

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