In vitro models in Alzheimer disease therapy

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Chmelyuk NS, Vadekhina VV, Ivanova AV, et al. [In vitro models in Alzheimer disease therapy]. Rossiiskii psikhiatricheskii zhurnal [Russian Journal of Psychiatry]. 2023;(6):31-40. Russian

Abstract

The main approaches to in vitro modeling of Alzheimer's disease, the possibility of their use in different conditions and the main advantages and disadvantages were presented in this scientific review with the aim of systematizing the available methods. Alzheimer's disease is one of the most complex diseases affecting the central nervous system. One of the most popular theories about the causes of Alzheimer's disease is the amyloid theory, which substantiates the degeneration of neurons due to the formation of amyloid beta (Aβ) plaques. In this regard, at the moment there is a large number of developments in the search for drugs aimed at destroying Aβ aggregates, which leads to attempts to create the most adequate in vitro models of the disease, allowing for rapid testing of new compounds. These may include methods based on the ability of Aβ to aggregate in water-salt buffers and bind to various dyes, such as Thioflavin T, Congo Red, Nile Red, or more complex models using cell lines and cultures.

Keywords Alzheimer's disease; beta-amyloid; fibrils; Thioflavin-T; in vitro model; central nervous system; neurodegenerative diseases

References

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DOI: http://dx.doi.org/10.34757/1560-957X.2023.27.6.004

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